Construct validation
Ensuring Robust and Reliable Peptide Presentation Analysis
IMMUNESPEC
Ensuring Robust and Reliable Peptide Presentation Analysis
Construct validation is a critical step in the development of DNA-, RNA-, and protein-based immunotherapies and vaccines. It ensures that engineered constructs properly encode, process, and present the intended antigens via major histocompatibility complex (MHC) molecules, ultimately enabling a desired immune response while minimizing unintended immunogenic effects.
By loading constructs onto antigen-presenting cells (APCs) in vitro and experimentally validating MHC presentation, developers can verify the biological relevance and functionality of their therapeutic constructs early in development. Given the complexity of antigen processing and presentation, experimental validation is essential to reduce development risk and guide optimization.
IMMUNESPEC
Comprehensive Construct Validation with Immunopeptidomics
Key benefits of immunopeptidomics-based construct validation:
✔ Confirms translation and proper processing of nucleic acid-encoded proteins
✔ Directly identifies MHC-bound peptides, validating intended antigen display
✔ Supports sequence optimization to enhance immunogenicity and reduce off-target effects
✔ Improves construct design early, reducing risk of late-stage clinical failure
This comprehensive view of presented peptides enables researchers to fine-tune constructs for maximum efficacy—supporting the development of safer, more effective vaccines and immunotherapies.
ImmuneSpec – Your Partner in Construct Validation
At ImmuneSpec, we offer advanced immunopeptidomics services tailored for construct validation. Whether you’re developing mRNA vaccines, viral vectors, or protein therapeutics, our platform provides the experimental clarity you need to move forward with confidence.
- Experimental validation of antigen processing and presentation: We provide direct identification of construct-derived peptides naturally presented via MHC class I and II molecules.
- Customizable workflows for transduced or transfected cells:
We work with both client-supplied and internally prepared APCs—supporting flexible integration with your construct development pipeline. - Detection of modified, cryptic, and non-canonical peptides:
We capture a full spectrum of presented epitopes, including those arising from alternative processing events and post-translational modifications. - Early insight to guide design:
Our data supports rational construct optimization, reducing the risk of downstream failure and improving therapeutic performance. - Validate with confidence:
Partner with ImmuneSpec to ensure your constructs present the right peptides—and deliver the immune response you intend.